WebNov 24, 2024 · A method to obtain bright dual-color photoconvertible fluorophores is presented based on a new mechanism called Directed Photooxidation Induced Conversion (DPIC). The mechanism is based on the directed oxidation of the pyrrole that is conjugated to the fluorophore. This approach led to SC-P, which stains the lipid droplets in live cells … WebMar 5, 2024 · Photoconvertible fluorophores are important for a myriad of applications in chemistry and biology. Here, we spectrally resolve and quantify individual photophysical information of a dual-emitting photoconvertible fluorophore by fluorescence spectroscopy and multivariate curve resolution-alternate least square techniques. We found that the ...
Brightfield image (a), overlap of three fluorescence channels (b), …
WebMar 5, 2024 · Photoconvertible fluorophores are important for a myriad of applications in chemistry and biology. Here, we spectrally resolve and quantify individual photophysical … WebSulfoxide-bridged dimeric BODIPYs were developed as a new class of long-wavelength photoconvertible fluorophores. Upon visible-light irradiation, a sulfoxide moiety was released to generate the ... incarnation\\u0027s ox
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WebNov 22, 2024 · Sulfoxide-bridged dimeric BODIPYs were developed as a new class of long-wavelength photoconvertible fluorophores. Upon visible-light irradiation, a sulfoxide moiety was released to generate the ... WebFeb 18, 2014 · Milder conditions are apparently needed to avoid phototoxic effects. Therefore, we turned our attention to a photoconvertible fluorophore, Dendra2 (Gurskaya et al., 2006; Chudakov et al., 2007). Dendra2 is a monomeric protein with a green-to-red photoconversion upon blue light exposure (Gurskaya et al., 2006). WebNov 24, 2024 · fluorophores, would enable to extend the palette of proper-ties and available colors of DCPFs for various applications in bioimaging. We thus aimed at establishing a rational design of DCPFs supported by a new mechanism. Herein, we introduce a strategy to obtain photoconvertible fluorophores based on Directed Photooxidation Induced … inclusive books for classroom