Phosphinothricin ppt
Web【技术实现步骤摘要】 一种D ‑ 氨基酸氧化酶突变体及其应用 : [0001]本专利技术涉及蛋白质工程领域,涉及一种D ‑ 氨基酸氧化酶突变体及其应用。 技术介绍 : [0002]草铵膦(glufosinate ammonium,phosphinothricin简称PPT),化学名称为(D,L) ‑2‑ 氨基 ‑4‑ [羟基(甲基)膦酰基]丁酸。 其分子式为:C5H 12 NO4P ... WebAug 3, 2024 · To determine the structure of Hpy GS, crystals of the apo- and substrate-bound forms in complex with ATP and phosphinothricin (PPT) were obtained. The apo structure of Hpy GS (Hpy GS apo S ...
Phosphinothricin ppt
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WebJan 1, 1995 · The bialaphos resistance gene (bar) is part of the biosynthesis pathway of bialaphos 6.The strains Streptomyces hygroscopicus and Streptomyces … WebOct 5, 2024 · Green Fluorescent Protein notes.ppt KimEliakim1 ... Sulfadiazine/asulam Dihydrofolate reductase (dhfr) Methotrexate Phosphinothricin acetyl transferase L-phosphinothricin (PPT), bialaphos 5-enolpyruvyl shikimate 3-phosphate (EPSP) synthase (aroA) Glyphosate (Roundup) Acetolactate synthase mutamt form (csr1-1) Sulphonylurea, …
WebPhosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only … WebJan 7, 2024 · In BY-2 cells, phosphinothricin had been already used by Nakamura et al. ( 2010 ), who recommended 2 mg/l PPT for selection. However, this protocol did not work in our hands, and later on, PPT was even considered unsuitable for suspension cultures by some authors (Seifertová et al. 2014 ).
WebDec 1, 1992 · Transgenic friable, embryogenic oat callus cultures were selected for phosphinothricin (PPT) resistance following microprojectile bombardment with a plasmid encoding the Streptomyces hygroscopicus ... WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am …
WebAug 16, 2007 · The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase ( pat) gene as a selectable marker and 5.0 mg l −1 of bialaphos as the selective agent.
WebPhosphinothricin (PPT) is the active ingredient in bialaphos, which specifically inhibits glutamine synthetase in land plants. We isolated a novel PPT-resistant gene from a soil … photo of coffee plantationWebAbout 50 =B5M phosphinothricin (PPT, the active ingredient in Basta) is sufficient for the selection *** I used pure phosphinotricin for selection of BASTA resistant Arabidopsis seedlings harboring a p35S-bar gene. This worked nicely at a concentration of 0.05 mM. photo of cold soreWebApr 1, 2024 · Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of … photo of clown fishWebJan 1, 1995 · It consists of phosphinothricin (PPT), which is an irreversible inhibitor of glutamine synthetase (glutamate–ammonia ligase), and two l-alanine residues. Glufosinate ammonium, the chemically synthesized PPT, and bialaphos are used as … how does low income affect childrenWebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to the ammonium salt of l-phosphinothricin (PPT, also called glufosinate), an irreversible inhibitor of glutamine synthetase activity and the main active component of the herbicide BASTA (AgrEvo, Frankfurt am Main, Germany). Under the same conditions, however, this strain … photo of college studentWebJan 12, 2024 · Abstract. Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed … how does low income affect healthWebMSDS Certificate of Analysis DL-Phosphinothricin (PPT) is an analogue of glutamate and acts as a competitive inhibi-tor of glutamine synthetase. This enzyme is involved in assimilation of ammonia and plays a key role in nitrogen assimilation. P0159.0250 250 mg € 94.20 Order P0159.1000 1 g € 331.70 Order * All prices are ex-works Haarlem NL. how does low income affect families