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High 5 tagged
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Web1 de ago. de 2007 · Individually tagged samples are combined into a single master library and treated with phosphatase. Half of the adapter is removed by SrfI digestion (V) leaving ligatable ends for the standard 454 library production. After sequencing (VI), the tags are used to identify the sample origin and removed prior to further analysis. WebAnti-His Tag antibodies enable the detection of the tagged proteins allowing screening of expression. The poly-His Tag is usually formed of 6 histidine residues but may be between three and ten, usually at the C- or N-terminus of the recombinant protein. Here we demonstrate the utility of Jackson ImmunoResearch’s Anti-His Tag antibodies for ...
Web5-tagged proteins can be purified by cation exchange resin SP-Sephadex, and most of the contami-nating proteins do not bind. After binding, the tagged proteins are eluted with a linear NaCl gradient at alkaline pH. Polyarginine might affect the tertiary structure of proteins whose C-terminal region is hydrophobic (Sassen-feld and Brewer 1984). WebHigh 5 Casino is your one-stop web destination for real Vegas hits! Experience the ultimate casino gameplay that offers you access to High 5 Games' social apps with exclusive …
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