Web2. Knowing your instrument. This is the second biggest factor (#1 is the first) in designing an accurate and effective antibody panel. First, you have to know the instrument configuration, which means you have to: … WebSecondary antibodies indirectly detect a primary antibody that specifically binds to the protein of interest. They can be conjugated to fluorophores (eg Alexa Fluor® dyes), enzymes (eg HRP, alkaline phosphatase), or proteins (eg biotin) to amplify the signal of primary antibodies in a wide range of applications including western blot, immunohistochemistry, …
Flow Cytometry Reagents - BioLegend
WebMay 10, 2024 · The optimal antibody concentration, which gives the best staining with minimum background, must be determined experimentally for each assay and is usually determined using a series of dilutions in a titration experiment. For example, if a product datasheet suggests using a 1:200 dilution, it is recommended to make dilutions of 1:50, … WebProduct Description. ExoBrite™ CD63 Flow Antibody is validated by Biotium for optimal detection of exosome marker CD63 in purified or bead-bound exosomes by flow cytometry. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, and PE channels. Optimal detection of exosome marker ... iredell inspections department
Flow Cytometry Antibodies - Sigma-Aldrich
WebAntibodies that detect CD16/CD32 can be used in several scientific applications, including Flow Cytometry, ELISA, Functional assay, Immunohistochemistry and … WebMany antibody clones are conjugated to a wide array of fluorophores, including Brilliant Violet™ dyes, KIRAVIA™ dyes, and our proprietary fluorophores, Spark and Fire Dyes. … WebFlow cytometer instruments, antibodies, and reagents from Bio-Rad give you the confidence to do more. The ZE5 Cell Analyzer is a flow cytometer that lets you expand your panels to look at more markers in your precious samples and run automated large-scale screening and validation experiments. The compact S3e Cell Sorter is a flow cytometer … order hockey sticks online